Reproductive and Developmental Biology, Genetics and Cytogenetics, Cryopreservation

Kuroda, M., T. Fujimoto, M. Murakami, E. Yamaha, K. Arai (2018) Clonal reproduction assured by sister chromosome pairing in dojo loach, a teleost fish. Chromosome Research, Online first, DOI: 10.1007/s10577-018-9581-4



Polonis, M., T. Fujimoto, S. Dobosz, T. Zalewski, K. Ocalewicz (2018) Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis. Journal of Applied Genetics, 59 (1): 91-97, DOI: 10.1007/s13353-017-0425-2



Endoh, M., T. Fujimoto, E. Yamaha and K. Arai (2018) Improved procedure for induction of the androgenetic doubled haploids in zebrafish. Zebrafish, 15: 33-44, DOI: 10.1089/zeb.2017.1482



Fujimoto, T., Yamada, A., Kodo, Y., Nakaya, K., Okubo-Murata, M., Saito, T., Ninomiya, K., Inaba, M., Kuroda, M., Arai, K., Murakami, M. (2017) Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species. Fisheries Science, 83: 743-756, DOI: 10.1007/s12562-017-1108-y



Havelka, M., T. Fujimoto, S. Hagihara, S. Adachi and K. Arai (2017) Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid. Scientific Reports, 7: 1694, DOI:10.1038/s41598-017-01768-3



Nynca, J., Judycka, S., Liszewska, E., Dobosz, S., Grudniewska, J., Arai, K., Fujimoto T., Ciereszko, A. (2016) Utility of different sugar extenders for cryopreservation and post-thaw storage of sperm from Salmonidae species. Aquaculture, 464: 340-348, DOI: 10.1016/j.aquaculture.2016.07.014



Yamada, A.,Y. Kodo, M. Murakami, M. Kuroda, T. Aoki, T. Fujimoto, and K. Arai (2015) Hybrid origin of gynogenetic clones and the introgression of their mitochondrial genome into sexual diploids through meiotic hybridogenesis in the loach, Misgurnus anguillicaudatus. Journal of Experimental Zoology Part A, 323A: 593-606, DOI: 10.1002/jez.1950



Hou, J., T. Fujimoto, T. Saito, E. Yamaha and K. Arai (2015) Generation of clonal zebrafish line by androgenesis without egg irradiation. Scientific Reports, 5: 13346, DOI: 10.1038/srep13346



Marín, A, T. Fujimoto and K. Arai (2013) Rapid species identification of fresh and processed scallops by multiplex PCR. Food Control, 32: 472-476, DOI: 10.1016/j.foodcont.2013.01.0 07



Inoue, D., T. Fujimoto, Y. Kawakami, G.S. Yasui, E. Yamaha and K. Arai (2012) Vitrification of primordial germ cells using whole embryos for gene-banking in loach, Misgurnus anguillicaudatus. Journal of Applied Ichthyology, 28: 919-924, DOI: 10.1111/jai.12058



Kawakami, Y, M. Ishihara, T. Saito, T. Fujimoto, S. Adachi, K. Arai, and E. Yamaha (2012) Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3' untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos. Journal of Animal Science, 90: 4256-4265, DOI: 10.2527/jas2011-4884



Yasui, G.S., T Fujimoto, L. Arias-Rodriguez, Y. Takagi and K. Arai (2012) Effects of ions and cryoprotective agents on sperm motility and fertilization success of Misgurnus anguillicaudatus eggs. Aquaculture, 344-349: 147-152, DOI: 10.1016/j.aquaculture.2012.03.005



Goto, R., T. Saito, T. Takeda, T. Fujimoto, M. Takagi, K. Arai and E. Yamaha (2012) Germ cells are not the primary factor for sexual fate determination in goldfish. Developmental Biology, 370: 98-109, DOI: 10.1016/j.ydbio.2012.07.010



Morishima, K., T. Fujimoto, M. Sato, A. Kawae, Y. Zhao, E. Yamaha and K. Arai (2011) Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish. BMC Biotechnology, 11:116, DOI: 10.1186/1472-6750-11-116



Yasui G. S., T. Fujimoto, S. Sakao, E. Yamaha and K. Arai (2011) Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres. Journal of Animal Science, 89: 2380-2388, DOI: 10.2527/jas.2010-3633



Fujimoto, T., T. Nishimura, R. Goto-Kazeto, Y. Kawakami, E. Yamaha and K. Arai (2010) Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish. Proceedings of National Academy of Science USA, 107: 17211-17216, DOI: 10.1073/pnas.1007032107



Saito, T., R. Goto-Kazeto, T. Fujimoto, Y. Kawakami, K. Arai and E. Yamaha (2010) Inter-species transplantation and migration of primordial germ cells in cyprinid fish. International Journal of Developmental Biology, 54: 1481-1486, DOI: 10.1387/ijdb.103111ts



Kawakami, Y., R. Goto-Kazeto, T. Saito, T. Fujimoto, S. Higaki, Y. Takahashi, K. Arai and E. Yamaha (2010) Generation of germ-line chimera zebrafish using primordial germ cells isolated from cultured blastomeres and cryopreserved embryoids. International Journal of Developmental Biology, 54: 1493-1501, DOI: 10.1387/ijdb.093059yk



Fujimoto, T., T. Saito, S. Sakao, K. Arai and E. Yamaha (2010) Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm. International Journal of Developmental Biology, 54: 827-835, DOI: 10.1387/ijdb.092896tf



Fujimoto, T., G. S. Yasui, M. Hayakawa, S. Sakao, E. Yamaha and K. Arai (2010) Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa of a natural tetraploid male. Aquaculture, 308: S133-139, DOI: 10.1016/j.aquaculture.2010.04.029



Yasui, G. S., T. Fujimoto and K. Arai (2010) Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis. Aquaculture, 308: S140-144, DOI: 10.1016/j.aquaculture.2010.05.041

Developmental engineering

Cryopreservation of germplasm

Germ cells

Atypical reproduction

Clone

Chromosome set manipulation

Hybrid

Meiosis

B.Sc. Marine Biological Science (No. 1849) Hokkaido University 2000

M. Sc. Marine Bioscience (No. 25386) Hokkaido University 2002

Ph.D. (Marine Bioscience) (No, A7377) Hokkaido University 2005

We can find some species with atypical reproductive mode, including clonal reproduction, gynogenesis, hybridogenesis etc., in nature. The molecular mechanism of atypical reproduction, however, is still unclear. The atypical reproduction will be useful tools for genetic improvement and seeding production for aquaculture. Thus, we try to clarify the mechanisms using loach Misgurnus anguillicaudatus, including a clonal population with a gynogenetic reproductive lineage in nature where bisexual reproduction mode is normally observed, and we would like to apply the mechanism for producing genetic identical gametes for seedling production in aquaculture. Furthermore, we induce artificial hybridizations using Cyprinid and Salmonid fish to examine the reproductive characteristics of the resultant hybrids. On the other hand, we also develop techniques of cryopreservation for gene banking and reconstitution of genotypes from cryopreserved germplasm. Second, we also develop techniques of gamete production using chromosome set manipulation and developmental engineering. Gametes are derived from germinal stem cells. The germinal stem cells are differentiated from primordial germ cells which are arisen during embryogenesis. Furthermore, the primordial germ cells are originated from a fertilized egg. Thus, we try to manipulate genetic information and chromosome sets of primordial germ cells, and try to produce genetically manipulated gametes derived from the primordial germ cells via germline chimera called “Surrogate propagation.”