北海道大学 大学院水産科学研究院 大学院水産科学院 水産学部

英語サイトへ 北海道大学
助教
田中 啓之
たなか ひろゆき
  • 海洋応用生命科学部門
  • 増殖生命科学科
  • 海洋生物工学講座
オフィス
管理研究棟602
メール
tanaka-h◎fish.hokudai.ac.jp
※「◎」を「@」に変更
電話
0138-40-8809
研究課題
(1) 無脊椎動物の筋肉タンパク質に関する研究
(2) 刺胞動物の筋収縮および筋収縮調節の分子機構解明
代表的な業績
Amino acid sequence of troponin C from scallop striated adductor muscle. 1994 J. Biol. Chem. 269, 3464–3468. Nishita K, Tanaka H, and Ojima T.

Amino acid sequence of troponin-I from Akazara scallop striated adductor muscle. 1998 J. Biochem. 124, 304–310. Tanaka H, Ojima T, and Nishita K.

Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I. 2005 FEBS J. 272, 4475–4486. Tanaka H, Takeya Y, Doi T, Yumoto F, Tanokura M, Ohtsuki I, Nishita K, and Ojima T. (https://doi.org/10.1111/j.1742-4658.2005.04866.x)

Spectroscopic and ITC study of the conformational change upon Ca2+-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle. 2008 Biochem. Biophys. Res. Commun.   369, 109–114. Yumoto F, Tanaka H, Nagata K, Miyauchi Y, Miyakawa T, Ojima T, and Tanokura M. (https://doi.org/10.1016/j.bbrc.2007.11.124)

Structure–function relationships of molluscan troponin T revealed by limited proteolysis. 2008 Biochim. Biophys. Acta-Proteins and Proteomics 1784, 1037–1042. Tanaka H, Suzuki H, Ohtsuki I, and Ojima T. (https://doi.org/10.1016/j.bbapap.2008.04.001)

Protein kinase A-dependent modulation of Ca2+ sensitivity in cardiac and fast skeletal muscles after reconstitution with cardiac troponin. 2009 J. Gen. Physiol. 133, 571–581. Matsuba D, Terui T, O-uchi J, Tanaka H, Ojima T, Ohtsuki I, Ishiwata S, Kurihara S, and Fukuda N. (https://doi.org/10.1085/jgp.200910206)
    
Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai. 2010 Comp. Biochem. Physiol., Part B   157, 317–325. Rahman M M, Inoue A, Tanaka H, and Ojima T. (https://doi.org/10.1016/j.cbpb.2010.07.006)

Proteomic analysis of inviable salmonid hybrids between female masu salmon Oncorhynchus masou masou and male rainbow trout Oncorhynchus mykiss during early embryogenesis. 2011 J. Fish Biol. 78, 1508–1528. Zheng, L, Tanaka, H, and Abe, S.
(https://doi.org/10.1111/j.1095-8649.2011.02958.x)

Structure of the Ca2+-saturated C-terminal domain of scallop troponin C in complex with a troponin I fragment. 2013 Biol. Chem. 394, 555–568. Kato YS, Yumoto F, Tanaka H, Miyakawa T, Miyauchi Y, Takeshita D, Sawano Y, Ojima T, Ohtsuki I, and Tanokura M. (https://doi.org/10.1515/hsz-2012-0152)

Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV. 2013 FEBS Lett. 587, 2612–2616. Tanaka H, Takahashi H, and Ojima T. (https://doi.org/10.1016/j.febslet.2013.06.042)

Troponin-I is present as an essential component of muscles in echinoderm larvae. 2017 Sci. Rep. 7, 43563. Yaguchi, S, Yaguchi J, and Tanaka H. (https://doi.org/10.1038/srep43563)

Smooth muscle-like Ca2+-regulation of actin-myosin interaction in adult jellyfish striated muscle. 2018 Sci. Rep. 8, 7776. Tanaka H, Ishimaru S, Nagatsuka Y, and Ohashi K. (https://doi.org/10.1038/s41598-018-24817-x)
担当授業
学部
基礎生命科学実験(分担)
水圏生化学実験(分担)
教員からのメッセージ
海洋生物は、低温や高水圧、高塩分などの陸上生物とは異なる環境で生命活動を行えるよう体の形態や生態を進化させてきました。細胞や細胞内の物質についても同様で、塩類の排出能力の高い細胞群や、低温でも効率的に機能できる酵素が生命を維持しています。運動器官である筋肉には独特の生態を支える様々な特徴があり、例えば、二枚貝の貝柱は、ヒトデなどの外敵にこじ開けられないように、エネルギーの消費なしに収縮状態を持続できる仕組みをもっています。私たちは、水産動物の細胞内で働くタンパク質を研究し、こうした生命現象・機能の仕組みを分子レベルで解明することを志しています。
最近、力を入れているのは、クラゲに代表される刺胞動物の研究です。刺胞動物は約6億年前、神経と筋肉を進化史上最初に獲得したとされています。しかし筋肉を構成するタンパク質の研究がほとんど行われてこなかったため、神経刺激に応じてアクチンとミオシンの相互作用が制御される仕組みが不明です。現在その解明を目指して、クラゲの傘を拍動させる筋肉からタンパク質を精製し機能や構造を調べています。クラゲやタンパク質の研究に関心のある方は、ぜひ当研究室を訪ねてみてください。
北海道大学研究者総覧システムへ
TANAKA Hiroyuki
  • Assistant Professor of Marine Biotechnology and Microbiology
  • Division of Marine Life Science
  • Department of Aquaculture Life Science
Office location
602 Main Building
Email
tanaka-h[at]fish.hokudai.ac.jp
※ change [at] to @
Phone
+81-138-40-8809
Research Areas
Proteins from marine invertebrates
Molecular regulatory mechanism of muscular contraction
Representative publications
Amino acid sequence of troponin C from scallop striated adductor muscle. 1994 J. Biol. Chem. 269, 3464–3468. Nishita K, Tanaka H, and Ojima T.

Amino acid sequence of troponin-I from Akazara scallop striated adductor muscle. 1998 J. Biochem. 124, 304–310. Tanaka H, Ojima T, and Nishita K.

Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I. 2005 FEBS J. 272, 4475–4486. Tanaka H, Takeya Y, Doi T, Yumoto F, Tanokura M, Ohtsuki I, Nishita K, and Ojima T. (https://doi.org/10.1111/j.1742-4658.2005.04866.x)

Spectroscopic and ITC study of the conformational change upon Ca2+-binding in TnC C-lobe and TnI peptide complex from Akazara scallop striated muscle. 2008 Biochem. Biophys. Res. Commun.   369, 109–114. Yumoto F, Tanaka H, Nagata K, Miyauchi Y, Miyakawa T, Ojima T, and Tanokura M. (https://doi.org/10.1016/j.bbrc.2007.11.124)

Structure–function relationships of molluscan troponin T revealed by limited proteolysis. 2008 Biochim. Biophys. Acta-Proteins and Proteomics 1784, 1037–1042. Tanaka H, Suzuki H, Ohtsuki I, and Ojima T. (https://doi.org/10.1016/j.bbapap.2008.04.001)

Protein kinase A-dependent modulation of Ca2+ sensitivity in cardiac and fast skeletal muscles after reconstitution with cardiac troponin. 2009 J. Gen. Physiol. 133, 571–581. Matsuba D, Terui T, O-uchi J, Tanaka H, Ojima T, Ohtsuki I, Ishiwata S, Kurihara S, and Fukuda N. (https://doi.org/10.1085/jgp.200910206)
    
Isolation and characterization of two alginate lyase isozymes, AkAly28 and AkAly33, from the common sea hare Aplysia kurodai. 2010 Comp. Biochem. Physiol., Part B   157, 317–325. Rahman M M, Inoue A, Tanaka H, and Ojima T. (https://doi.org/10.1016/j.cbpb.2010.07.006)

Proteomic analysis of inviable salmonid hybrids between female masu salmon Oncorhynchus masou masou and male rainbow trout Oncorhynchus mykiss during early embryogenesis. 2011 J. Fish Biol. 78, 1508–1528. Zheng, L, Tanaka, H, and Abe, S. (https://doi.org/10.1111/j.1095-8649.2011.02958.x)

Structure of the Ca2+-saturated C-terminal domain of scallop troponin C in complex with a troponin I fragment. 2013 Biol. Chem. 394, 555–568. Kato YS, Yumoto F, Tanaka H, Miyakawa T, Miyauchi Y, Takeshita D, Sawano Y, Ojima T, Ohtsuki I, and Tanokura M. (https://doi.org/10.1515/hsz-2012-0152)

Ca2+-binding properties and regulatory roles of lobster troponin C sites II and IV. 2013 FEBS Lett. 587, 2612–2616. Tanaka H, Takahashi H, and Ojima T. (https://doi.org/10.1016/j.febslet.2013.06.042)

Troponin-I is present as an essential component of muscles in echinoderm larvae. 2017 Sci. Rep. 7, 43563. Yaguchi, S, Yaguchi J, and Tanaka H. (https://doi.org/10.1038/srep43563)

Smooth muscle-like Ca2+-regulation of actin-myosin interaction in adult jellyfish striated muscle. 2018 Sci. Rep. 8, 7776. Tanaka H, Ishimaru S, Nagatsuka Y, and Ohashi K. (https://doi.org/10.1038/s41598-018-24817-x)
HU Researcher Database