北海道大学 大学院水産科学研究院 大学院水産科学院 水産学部

英語サイトへ 北海道大学
准教授
藤本 貴史
ふじもと たかふみ
  • 海洋応用生命科学部門
  • 増殖生命科学科
  • 育種生物学講座
オフィス
管理研究棟402
メール
fujimoto◎fish.hokudai.ac.jp
※「◎」を「@」に変更
電話
0138-40-5536
研究課題
(1) クローンドジョウの配偶子形成機構の解明
(2) 発生工学的手法によるクローン配偶子生産技術の開発
(3) 雑種(サケ科魚類、コイ目魚類)の生殖特性
(4) 生殖細胞を用いた遺伝資源保存・再生技術の開発
代表的な業績
Kuroda, M., T. Fujimoto, M. Murakami, E. Yamaha, K. Arai (2018) Clonal reproduction assured by sister chromosome pairing in dojo loach, a teleost fish. Chromosome Research, Online first: xxx-xxx, DOI: 10.1007/s10577-018-9581-4

Polonis, M., T. Fujimoto, S. Dobosz, T. Zalewski, K. Ocalewicz (2018) Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis. Journal of Applied Genetics, 59 (1): 91-97, DOI: 10.1007/s13353-017-0425-2

Endoh, M., T. Fujimoto, E. Yamaha and K. Arai (2018) Improved procedure for induction of the androgenetic doubled haploids in zebrafish. Zebrafish, 15: 33-44, DOI: 10.1089/zeb.2017.1482

Fujimoto, T., Yamada, A., Kodo, Y., Nakaya, K., Okubo-Murata, M., Saito, T., Ninomiya, K., Inaba, M., Kuroda, M., Arai, K., Murakami, M. (2017) Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species. Fisheries Science, 83: 743-756, DOI: 10.1007/s12562-017-1108-y

Havelka, M., T. Fujimoto, S. Hagihara, S. Adachi and K. Arai (2017) Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid. Scientific Reports, 7: 1694, DOI:10.1038/s41598-017-01768-3

Nynca, J., Judycka, S., Liszewska, E., Dobosz, S., Grudniewska, J., Arai, K., Fujimoto T., Ciereszko, A. (2016) Utility of different sugar extenders for cryopreservation and post-thaw storage of sperm from Salmonidae species. Aquaculture, 464: 340-348, DOI: 10.1016/j.aquaculture.2016.07.014

Yamada, A.,Y. Kodo, M. Murakami, M. Kuroda, T. Aoki, T. Fujimoto, and K. Arai (2015) Hybrid origin of gynogenetic clones and the introgression of their mitochondrial genome into sexual diploids through meiotic hybridogenesis in the loach, Misgurnus anguillicaudatus. Journal of Experimental Zoology Part A, 323A: 593-606, DOI: 10.1002/jez.1950

Hou, J., T. Fujimoto, T. Saito, E. Yamaha and K. Arai (2015) Generation of clonal zebrafish line by androgenesis without egg irradiation. Scientific Reports, 5: 13346, DOI: 10.1038/srep13346

Marín, A, T. Fujimoto and K. Arai (2013) Rapid species identification of fresh and processed scallops by multiplex PCR. Food Control, 32: 472-476, DOI: 10.1016/j.foodcont.2013.01.0 07

Inoue, D., T. Fujimoto, Y. Kawakami, G.S. Yasui, E. Yamaha and K. Arai (2012) Vitrification of primordial germ cells using whole embryos for gene-banking in loach, Misgurnus anguillicaudatus. Journal of Applied Ichthyology, 28: 919-924, DOI: 10.1111/jai.12058

Kawakami, Y, M. Ishihara, T. Saito, T. Fujimoto, S. Adachi, K. Arai, and E. Yamaha (2012) Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3' untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos. Journal of Animal Science, 90: 4256-4265, DOI: 10.2527/jas2011-4884

Yasui, G.S., T Fujimoto, L. Arias-Rodriguez, Y. Takagi and K. Arai (2012) Effects of ions and cryoprotective agents on sperm motility and fertilization success of Misgurnus anguillicaudatus eggs. Aquaculture, 344-349: 147-152, DOI: 10.1016/j.aquaculture.2012.03.005

Goto, R., T. Saito, T. Takeda, T. Fujimoto, M. Takagi, K. Arai and E. Yamaha (2012) Germ cells are not the primary factor for sexual fate determination in goldfish. Developmental Biology, 370: 98-109, DOI: 10.1016/j.ydbio.2012.07.010

Morishima, K., T. Fujimoto, M. Sato, A. Kawae, Y. Zhao, E. Yamaha and K. Arai (2011) Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish. BMC Biotechnology, 11:116, DOI: 10.1186/1472-6750-11-116

Yasui G. S., T. Fujimoto, S. Sakao, E. Yamaha and K. Arai (2011) Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres. Journal of Animal Science, 89: 2380-2388, DOI: 10.2527/jas.2010-3633

Fujimoto, T., T. Nishimura, R. Goto-Kazeto, Y. Kawakami, E. Yamaha and K. Arai (2010) Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish. Proceedings of National Academy of Science USA, 107: 17211-17216, DOI: 10.1073/pnas.1007032107

Saito, T., R. Goto-Kazeto, T. Fujimoto, Y. Kawakami, K. Arai and E. Yamaha (2010) Inter-species transplantation and migration of primordial germ cells in cyprinid fish. International Journal of Developmental Biology, 54: 1481-1486, DOI: 10.1387/ijdb.103111ts

Kawakami, Y., R. Goto-Kazeto, T. Saito, T. Fujimoto, S. Higaki, Y. Takahashi, K. Arai and E. Yamaha (2010) Generation of germ-line chimera zebrafish using primordial germ cells isolated from cultured blastomeres and cryopreserved embryoids. International Journal of Developmental Biology, 54: 1493-1501, DOI: 10.1387/ijdb.093059yk

Fujimoto, T., T. Saito, S. Sakao, K. Arai and E. Yamaha (2010) Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm. International Journal of Developmental Biology, 54: 827-835, DOI: 10.1387/ijdb.092896tf

Fujimoto, T., G. S. Yasui, M. Hayakawa, S. Sakao, E. Yamaha and K. Arai (2010) Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa of a natural tetraploid male. Aquaculture, 308: S133-139, DOI: 10.1016/j.aquaculture.2010.04.029

Yasui, G. S., T. Fujimoto and K. Arai (2010) Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis. Aquaculture, 308: S140-144, DOI: 10.1016/j.aquaculture.2010.05.041
担当授業
学部
水族発生生物学(科目担当者)
水族遺伝育種学(科目担当者,分担)
発生・組織学(分担)
水産増殖学(分担)
水産科学英語I, II(分担)
サケ学入門(分担)
水圏生物学実験(分担)
増養殖実習(分担)
潜水実習(分担)

大学院
育種生物学特論I, II(分担)
水産科学汎論I(分担)
略歴
2000年3月 北海道大学 水産学部 生物生産科学科
2002年3月 北海道大学 大学院水産科学研究科 生命資源科学専攻博士前期(修士)課程
2005年3月 北海道大学 大学院水産科学研究科 生命資源科学専攻博士後期課程
2005年4月~2005年5月 北海道大学大学院水産科学研究院  学術研究員
2005年6月~2008年3月 北海道大学大学院水産科学研究院  博士研究員2008年4月 - 2008年4月~2010年8月 北海道大学北方生物圏フィールド科学センター  博士研究員
2010年9月~2012年3月 カナダ 水産海洋省  日本学術振興会海外特別研究員
2012年4月~現在 北海道大学 大学院水産科学研究院 海洋応用生命科学部門 育種生物学分野  准教授
受賞
2010年 平成21年度 日本水産学会論文賞
2017年	平成28年度 日本水産学会進歩賞
社会貢献
文部科学省 科学技術・学術政策研究所 専門調査員
教員からのメッセージ
生物生産の基本は配偶子の生産であり、育種の最終目標は様々な消費者と生産者のニーズに適った様々な特性を兼ね備えた魚介類を創出することにあります。育種生物学分野の私たちの研究室では配偶子に様々な遺伝的な特性を付与することで、より優れた種苗を生産できると考え研究を行っています。配偶子は生殖細胞に起源し、胚発生期に遡れば始原生殖細胞、さらには受精卵そのものに辿り着きます。そこで、私たちは受精卵や始原生殖細胞、そして生殖細胞を材料に用い、ゲノム構成が減数分裂や配偶子形成に与える影響について、様々な角度から多種多様な方法を駆使して研究に取り組んでいます。また、多くの育種家や研究者が長い歳月をかけて生み出した系統を有効に保存・再生するための遺伝資源保存・再生技術の開発も行っています。
大学生活の四年間は諸君らが思う以上に短く、あっという間に終わってしまいます。この貴重な期間に、様々なことに挑戦し、よく学び、色々と考え、豊かな人間性を培って下さい。
北海道大学研究者総覧システムへ
FUJIMOTO Takafumi
  • Associate Professor of Laboratory of Aquaculture Genetics and Genomics Division of Marine Life Science
Office location
402 Main Building
Email
fujimoto[at]fish.hokudai.ac.jp
※ change [at] to @
Phone
+81-138-40-5536
Research Areas
Reproductive and Developmental Biology, Genetics and Cytogenetics, Cryopreservation
Representative publications
Kuroda, M., T. Fujimoto, M. Murakami, E. Yamaha, K. Arai (2018) Clonal reproduction assured by sister chromosome pairing in dojo loach, a teleost fish. Chromosome Research, Online first, DOI: 10.1007/s10577-018-9581-4

Polonis, M., T. Fujimoto, S. Dobosz, T. Zalewski, K. Ocalewicz (2018) Genome incompatibility between rainbow trout (Oncorhynchus mykiss) and sea trout (Salmo trutta) and induction of the interspecies gynogenesis. Journal of Applied Genetics, 59 (1): 91-97, DOI: 10.1007/s13353-017-0425-2

Endoh, M., T. Fujimoto, E. Yamaha and K. Arai (2018) Improved procedure for induction of the androgenetic doubled haploids in zebrafish. Zebrafish, 15: 33-44, DOI: 10.1089/zeb.2017.1482

Fujimoto, T., Yamada, A., Kodo, Y., Nakaya, K., Okubo-Murata, M., Saito, T., Ninomiya, K., Inaba, M., Kuroda, M., Arai, K., Murakami, M. (2017) Development of nuclear DNA markers to characterize genetically diverse groups of Misgurnus anguillicaudatus and its closely related species. Fisheries Science, 83: 743-756, DOI: 10.1007/s12562-017-1108-y

Havelka, M., T. Fujimoto, S. Hagihara, S. Adachi and K. Arai (2017) Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid. Scientific Reports, 7: 1694, DOI:10.1038/s41598-017-01768-3

Nynca, J., Judycka, S., Liszewska, E., Dobosz, S., Grudniewska, J., Arai, K., Fujimoto T., Ciereszko, A. (2016) Utility of different sugar extenders for cryopreservation and post-thaw storage of sperm from Salmonidae species. Aquaculture, 464: 340-348, DOI: 10.1016/j.aquaculture.2016.07.014

Yamada, A.,Y. Kodo, M. Murakami, M. Kuroda, T. Aoki, T. Fujimoto, and K. Arai (2015) Hybrid origin of gynogenetic clones and the introgression of their mitochondrial genome into sexual diploids through meiotic hybridogenesis in the loach, Misgurnus anguillicaudatus. Journal of Experimental Zoology Part A, 323A: 593-606, DOI: 10.1002/jez.1950

Hou, J., T. Fujimoto, T. Saito, E. Yamaha and K. Arai (2015) Generation of clonal zebrafish line by androgenesis without egg irradiation. Scientific Reports, 5: 13346, DOI: 10.1038/srep13346

Marín, A, T. Fujimoto and K. Arai (2013) Rapid species identification of fresh and processed scallops by multiplex PCR. Food Control, 32: 472-476, DOI: 10.1016/j.foodcont.2013.01.0 07

Inoue, D., T. Fujimoto, Y. Kawakami, G.S. Yasui, E. Yamaha and K. Arai (2012) Vitrification of primordial germ cells using whole embryos for gene-banking in loach, Misgurnus anguillicaudatus. Journal of Applied Ichthyology, 28: 919-924, DOI: 10.1111/jai.12058

Kawakami, Y, M. Ishihara, T. Saito, T. Fujimoto, S. Adachi, K. Arai, and E. Yamaha (2012) Cryopreservation of green fluorescent protein (GFP)-labeled primordial germ cells with GFP fused to the 3' untranslated region of the nanos gene by vitrification of Japanese eel (Anguilla japonica) somite stage embryos. Journal of Animal Science, 90: 4256-4265, DOI: 10.2527/jas2011-4884

Yasui, G.S., T Fujimoto, L. Arias-Rodriguez, Y. Takagi and K. Arai (2012) Effects of ions and cryoprotective agents on sperm motility and fertilization success of Misgurnus anguillicaudatus eggs. Aquaculture, 344-349: 147-152, DOI: 10.1016/j.aquaculture.2012.03.005

Goto, R., T. Saito, T. Takeda, T. Fujimoto, M. Takagi, K. Arai and E. Yamaha (2012) Germ cells are not the primary factor for sexual fate determination in goldfish. Developmental Biology, 370: 98-109, DOI: 10.1016/j.ydbio.2012.07.010

Morishima, K., T. Fujimoto, M. Sato, A. Kawae, Y. Zhao, E. Yamaha and K. Arai (2011) Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish. BMC Biotechnology, 11:116, DOI: 10.1186/1472-6750-11-116

Yasui G. S., T. Fujimoto, S. Sakao, E. Yamaha and K. Arai (2011) Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres. Journal of Animal Science, 89: 2380-2388, DOI: 10.2527/jas.2010-3633

Fujimoto, T., T. Nishimura, R. Goto-Kazeto, Y. Kawakami, E. Yamaha and K. Arai (2010) Sexual dimorphism of gonadal structure and gene expression in germ cell-deficient loach, a teleost fish. Proceedings of National Academy of Science USA, 107: 17211-17216, DOI: 10.1073/pnas.1007032107

Saito, T., R. Goto-Kazeto, T. Fujimoto, Y. Kawakami, K. Arai and E. Yamaha (2010) Inter-species transplantation and migration of primordial germ cells in cyprinid fish. International Journal of Developmental Biology, 54: 1481-1486, DOI: 10.1387/ijdb.103111ts

Kawakami, Y., R. Goto-Kazeto, T. Saito, T. Fujimoto, S. Higaki, Y. Takahashi, K. Arai and E. Yamaha (2010) Generation of germ-line chimera zebrafish using primordial germ cells isolated from cultured blastomeres and cryopreserved embryoids. International Journal of Developmental Biology, 54: 1493-1501, DOI: 10.1387/ijdb.093059yk

Fujimoto, T., T. Saito, S. Sakao, K. Arai and E. Yamaha (2010) Developmental potential of embryonic cells in a nucleocytoplasmic hybrid formed using a goldfish haploid nucleus and loach egg cytoplasm. International Journal of Developmental Biology, 54: 827-835, DOI: 10.1387/ijdb.092896tf

Fujimoto, T., G. S. Yasui, M. Hayakawa, S. Sakao, E. Yamaha and K. Arai (2010) Reproductive capacity of neo-tetraploid loaches produced using diploid spermatozoa of a natural tetraploid male. Aquaculture, 308: S133-139, DOI: 10.1016/j.aquaculture.2010.04.029

Yasui, G. S., T. Fujimoto and K. Arai (2010) Restoration of the loach, Misgurnus anguillicaudatus, from cryopreserved diploid sperm and induced androgenesis. Aquaculture, 308: S140-144, DOI: 10.1016/j.aquaculture.2010.05.041
Keywords
Developmental engineering
Cryopreservation of germplasm
Germ cells
Atypical reproduction
Clone
Chromosome set manipulation
Hybrid
Meiosis
Degrees:
B.Sc. Marine Biological Science (No. 1849) Hokkaido University 2000
M. Sc. Marine Bioscience (No. 25386) Hokkaido University 2002
Ph.D. (Marine Bioscience) (No, A7377) Hokkaido University 2005
Message
We can find some species with atypical reproductive mode, including clonal reproduction, gynogenesis, hybridogenesis etc., in nature. The molecular mechanism of atypical reproduction, however, is still unclear. The atypical reproduction will be useful tools for genetic improvement and seeding production for aquaculture. Thus, we try to clarify the mechanisms using loach Misgurnus anguillicaudatus, including a clonal population with a gynogenetic reproductive lineage in nature where bisexual reproduction mode is normally observed, and we would like to apply the mechanism for producing genetic identical gametes for seedling production in aquaculture. Furthermore, we induce artificial hybridizations using Cyprinid and Salmonid fish to examine the reproductive characteristics of the resultant hybrids. On the other hand, we also develop techniques of cryopreservation for gene banking and reconstitution of genotypes from cryopreserved germplasm. Second, we also develop techniques of gamete production using chromosome set manipulation and developmental engineering. Gametes are derived from germinal stem cells. The germinal stem cells are differentiated from primordial germ cells which are arisen during embryogenesis. Furthermore, the primordial germ cells are originated from a fertilized egg. Thus, we try to manipulate genetic information and chromosome sets of primordial germ cells, and try to produce genetically manipulated gametes derived from the primordial germ cells via germline chimera called “Surrogate propagation.”
HU Researcher Database